254191
NOX2/gp91phox (CGD91-phox, CYBB, Cytochrome b(558) beta chain, Cytochrome b-245 heavychain, gp91-1, gp91-phox, Heme-Binding Membrane Glycoprotein gp91phox, NADPHoxidase 2, Neutrophil cytochrome b 91kD Polypeptide, NOX2, p22 phagocyte B-cytochrome, Superoxide-generating NADPH oxidase heavy chain Subunit)
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£731.00
- SKU:
- 254191
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)
- Klon:
- 54.1
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Weitere Details:
- Cytochrome b-245 heavy chain (CYBB), which is also know as NOX2/gp91phox. NOX2/gp91phox is a component of the membrane-bound oxidase of phagocytes that generates superoxide. Defects in the CYBB gene are a cause of Chronic X-linked granulomatous disease (CGD). Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: Use at an assay dependent dilution. Detects a band of ~65kD (predicted molecular weight: 65kD). Immunocytochemistry/Immunofluorescence: 1ug/ml Immunohistochemistry(Paraffin): 5ug/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice