HEF1 (HEF1 (Cas-L), CASL, NEDD9, NEDD9 Protein, NY REN 12 antigen, p105, Protein NEDD9)

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£731.00

SKU:: 254080
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Fusion protein, corresponding to aa82-398 of Human HEF1.
Klon:: 2G9
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Weitere Details:: HEF1 is a multifunctional protein involved in integrin-based signaling that affects cell motility growth apoptosis and oncogenic transformation The Cas family of docking proteins have been the subject of intense research because of their role in cell motility growth apoptosis and oncogenic transformation These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases two active targets for drug development HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility cell transformation and invasiveness including several metalloproteinases MLCK p160ROCK and ErbBi HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines including MCF7 and HeLa cells Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas) a related protein is associated with tamoxifen resistance This highlights the importance of studying the role of this family of proteins in cancer prognosis. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000-1:6000. Predicted molecular weight: 93kD. Immunoprecipitation: 1:1000 Immunocytochemistry/Immunofluorescence: Use at an assay dependent dilution. PubMed: 19376971 Immunohistochemistry(Frozen): Use at an assay dependent dilution. PubMed: 19464348 Flow Cytometry: 1ug for 10e6 cells Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice