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227418-ML650

CD4 (MaxLight 650)

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£856.00

SKU:
227418-ML650
Reinheit:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Lagerbedingungen:
4°C Do Not Freeze
Hersteller:
United States Biological
Host:
Mouse
Immunogen:
Bovine lymphocytes.
Klon:
CC8
Format:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Weitere Details:
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Recognizes bovine CD4 and immunoprecipitates a molecule of ~50kD and considered to be the bovine homolog of human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Eskra et al. 1991). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Versandbedingungen:
Blue Ice