226791
Caspase-8 (Apoptotic Cysteine Protease, Apoptotic Protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like Protease)
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£479.00
- SKU:
- 226791
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to mouse caspase-8 (p20 subunit).
- Klon:
- 15B285
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Weitere Details:
- Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Endotoxin: <0.1EU/ug purified protein (LAL test; Lonza). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice