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Caspase-2 (CASP-2, Protease ICH-1, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 2, NEDD-2)
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£544.00
- SKU:
- 226789
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to human caspase-2 (p19 subunit).
- Klon:
- 10C6
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Weitere Details:
- Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase. Applications: Suitable for use in Flow Cytometry, ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen Immunocytochemistry: 10-20ug/ml. Fix in 4% paraformaldehyde, 0.18% Triton-X100 for 10 min prior to staining Optimal dilutions to be determined by the researcher. Endotoxin: <0.1EU/ug (LAL) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice