222842
GAD-65/67 (Glutamate Decarboxylase 2, 65kD Glutamic acid Decarboxylase, GAD 65, Glutamate Decarboxylase 65kD Isoform, GAD2, GAD-65, GAD 2, GAD-2, Glutamate Decarboxylase 1, 67kD Glutamic acid Decarboxylase, GAD 67, Glutamate Decarboxylase 67kD Isoform, GAD1, GAD, GAD67, GAD-1, GAD 1)
Größe
£672.00
- SKU:
- 222842
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to amino acids 540-590 of Human GAD-65.
- Format:
- Supplied as a liquid PBS, 0.02% sodium azide, pH 7.2 50% glycerol.
- Weitere Details:
- GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (y-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice