218722
IgG (Agarose)
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£499.00
- SKU:
- 218722
- Anwendung:
- IP
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Chicken/Avian
- Immunogen:
- Highly purified bovine IgG isolated
- Format:
- Supplied as a gel in PBS, pH 7.2, 0.05% sodium azide. Labeled with N-hydroxysuccinimide (NHS)-activated agarose beads.
- Weitere Details:
- Chickens were immunized with highly purified bovine IgG isolated from normal pooled bovine serum. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbant and conjugated to N-hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites. Applications: Suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Specific Activity: 1mg bovine IgG bound/ml agarose. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Note: We do not recommended adding glycerol as a cryopreservative.
- Versandbedingungen:
- Blue Ice