SSR1, Recombinant, Human, aa22-207, His-Tag (Translocon-Associated Protein Subunit alpha Precursor)

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£512.00

SKU:: 218585
Molekulargewicht:: 23.1
Reinheit:: ~90% (SDS-PAGE)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 20% glycerol.
Weitere Details:: The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34kD glycoprotein encoded by this gene and a 22kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. Recombinant was expressed in E.coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa22-207 from human SSR1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~23.1kD (209aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.