PRKAA1, Recombinant, Human, aa1-279, His-Tag (5-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1 Isoform 1, AMPK, AMPKa11)

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£512.00

SKU:: 218473
Molekulargewicht:: 34.3
Reinheit:: ≥85% (SDS-PAGE)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol.
Weitere Details:: PRKAA1 is also known as 5-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1. PRKAA1 is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinasen activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Source: Recombinant protein corresponding to aa1-279 from human PRKAA1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~34.3kD (302aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.