218469
PPM1D, Recombinant, Human, aa98-375, His-Tag (Protein Phosphatase 1D Magnesium-Dependent, Delta Isoform)
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£512.00
- SKU:
- 218469
- Molekulargewicht:
- 33.2
- Reinheit:
- ~90% (SDS-PAGE)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol.
- Weitere Details:
- PPM1D is also known as Protein phosphatase 1D magnesium-dependent, delta isoform. This protein is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase (MAPK/p38) through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. Source: Recombinant protein corresponding to aa98-375 from human PPM1D, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~33.2kD (299aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice