PpiA, Recombinant, Mouse, aa1-164, His-Tag (Peptidyl-Prolyl Cis-Trans Isomerase A, Cyclophilin A)

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£512.00

SKU:: 218468
Molekulargewicht:: 20.4
Reinheit:: ≥95% (SDS-PAGE)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 1mM DTT, 10% glycerol.
Weitere Details:: PpiA is also known as Peptidyl-prolyl cis-trans isomerase A. This is an enzyme found in both prokaryotes and eukaryotes that interconverts the cis and trans isomers of peptide bonds with the amino acid proline. Proline has an unusually conformationally restrained peptide bond due to its cyclic structure with its side chain bonded to its secondary amine nitrogen. Most amino acids have a strong energetic preference for the trans peptide bond conformation due to steric hindrance, but proline's unusual structure stabilizes the cis form so that both isomers are populated under biologically relevant conditions. Source: Recombinant protein corresponding to aa1-164 from mouse ppiA, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~20.4kD (187aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice