NSDHL, Recombinant, Human, aa1-297, His-Tag (Sterol-4-alpha-Carboxylate 3-Dehydrogenase, Decarboxylating)

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£512.00

SKU:: 218404
Molekulargewicht:: 35.5
Reinheit:: ≥80% (SDS-PAGE)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 10% glycerol.
Weitere Details:: NSDHL, also known as Sterol-4-alpha-carboxylate 3-dehydrogenase, is involved in the production (synthesis) of cholesterol. During cholesterol synthesis, it participates in one of several steps that convert a molecule called lanosterol to cholesterol. Specifically, this enzyme removes a carbon atom and three hydrogen atoms (a methyl group) in the conversion of lanosterol to cholesterol. It is also an important component of cell membranes and myelin, the fatty covering that insulates nerve cells. Source: Recombinant corresponding to aa1-297 from human NSDHL protein, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~35.5kD (320aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.