217902
Cytochrome P450 7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide, CYP7B1)
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£512.00
- SKU:
- 217902
- Reinheit:
- Supernatant
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Ovalbumin-conjugated synthetic peptide IQYPDSDVL (C-terminal sequence)
- Klon:
- M17-P3F2
- Format:
- Supplied as a liquid.
- Weitere Details:
- CYP7B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: Neat Western Blot: 1:2 Immunohistochemistry: Neat. Antigen retrieval: microwave 20 min @ 800W in 10mM citrate buffer, pH 6. Optimal dilutions to be determined by the researcher. Positive Control: Immunohistochemistry: formalin-fixed, paraffin-embedded human liver sections Western Blot: pooled human liver microsomes Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice