217662
PIM Kinase Inhibitor VIII, R8-T198wt (NH2-GGGRRRRRRRRGC~KKPGLRRRQT-CO2H, cpm-P27Kip1189-198)
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£519.00
- SKU:
- 217662
- Molekulargewicht:
- 2820.4
- Reinheit:
- ≥95% (HPLC)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a white solid.
- Weitere Details:
- The human p27Kip1 C-terminus PIM-1 phosphorylation site- (Thr198) containing sequence is synthesized with an N-terminal Arg octamer/R8-containing sequence to facilitate its use as a cell-permeable, substrate-competitive PIM-1 inhibitor. Reported to completely prevents human prostate carcinoma DU145-derived tumor expansion for up to 17 days (0.5umol/animal on days 0 & 5; intratumoral injection) in mice in vivo when combined with a single Taxol dosage on day zero (60mg/kg; i.p.). Studies using FITC-conjugated R8-T198wt demonstrate that R8-T198wt-induced cell cycle arrest and apoptosis in PIM-1-expressing DU145 cells are direct consequences of inhibition of PIM-1-mediated p27Kip1 Thr198 and Bad Ser112 phosphorylation, respectively. Formula: C₁₁₁H₂₁₁N₅₉O₂₆S₁ Sequence: H-Gly-Gly-Gly-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Cys-Lys-Lys-Pro-Gly-Leu-Arg-Arg-Arg-Gln-Thr-OH Molecular Weight: 2820.4 Solubility: DMSO (50mg/ml, clear, colorless solution) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice