NBD-Binding Peptide II, Cell Permeable (CTP-NBD, NEMO-Binding Domain Binding Peptide II, Cell-Permeable, CTP-NBD)

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£685.00

SKU:: 217579
Molekulargewicht:: 2846.2
Reinheit:: ~97% (HPLC)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a white solid.
Weitere Details:: A cell-permeable cytoplasmic transduction peptide (CTP; YGRRARRRARR) fused to the NEMO binding domain peptide (NBD; TALDWSWLQTE) that acts as a potent inhibitor of TNFa-stimulated NF-kB activity (IC50~50uM in HCT116 cells). Acts by interfering with the formation of active IKK complex and thereby prevents lkBa phosphorylation and its subsequent proteasomal degradation. Also blocks LPS-mediated increase in inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) activities. Colon-targeted capsules containing CTP-NBD are shown to reduce 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rodent models (4.5mg/kg/,p.o.) in vivo and diminish activities of myeloperoxidase and several inflammatory mediators. Formula: C₁₂₃H₁₉₇N₄₇O₃₂ Molecular Weight: 2846.2 Solubility: H2O (50mg/ml) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.