QTRT1 (FP3235, Guanine Insertion Enzyme, TGT, Catalytic Subunit, TGUT, TGT, 43-KD Subunit, TGT, TRNA-guanine Transglycosylase)

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£614.00

SKU:: 215790
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein fragment corresponding to the central region from human QTRT1. Species sequence homology: xenopus (tropicalis and laevis) and zebrafish
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.0, 0.01% Thimerosal, 20% glycerol.
Weitere Details:: tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD subunit (USP14; MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001 [PubMed 11255023]). Applications: Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:3000 Immunohistochemistry (Paraffin): 1:100-1:1000 Immunocytochemistry/Immunofluorescence: 1:100-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.