Ubiquitin C Terminal Hydrolase 1 (Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1, UCH-L1, Neuron Cytoplasmic Protein 9.5, PGP 9.5, PGP9.5, Ubiquitin Thioesterase L1, UCHL1)

Größe

£735.00

SKU:: 215503
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant full length protein corresponding to human Ubiquitin C Terminal Hydrolase purified from E. coli.
Klon:: BH7
Format:: Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.
Weitere Details:: This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:20,000 Immunohistochemistry: 1:500-1:2000 Immunocytochemistry: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.