215209
von Willebrand Factor (vWF, F8VWF, Factor VIII-Related Antigen)
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£793.00
- SKU:
- 215209
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human Factor VIII complex partially purified from Factor VIII concentrate.
- Klon:
- RFFVIII R/2
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Weitere Details:
- Von Willebrand factor (vWF), also known as Factor VIII related antigen, is a blood glycoprotein involved in blood coagulation. It stabilizes circulating Factor VIII by binding to it and protecting it from cleavage and delivers it to sites of vascular injury. vWF also promotes the adhesion of platelets to sites of vascular damage by forming a molecular bridge between collagen on exposed endothelial cells and the GPIb binding sites of platelets circulating in the blood. vWF circulates in the blood as large multimers, with each monomer (250kD) containing a number of specific domains. Hereditary or acquired defects in vWF lead to von Willebrand disease (vWD), characterised by varying degrees of susceptibility to bleeding. Symptoms might include nosebleeds, bleeding gums, easy bruising, menorrrhagia or gastrointestinal bleeding. Various forms of vWD exist with differing severities, determined by the type of defect. Clone RFFVIII R/2 has a high affinity for an epitope within the platelet GPIb-binding site that is responsible for biological activity. As such the antibody is a potent inhibitor of vWF activity. It can completely neutralise ristocetin-induced platelet aggregation and ristocetin-induced binding of vWF to platelets. It also inhibits platelet adhesion to glass beads. The epitope recognized is present only on the intact multimeric form of vWF and is abolished by mild denaturation with SDS. Applications: Suitable for use in RIA, ELISA, Immunohistochemistry and Protein Purification. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:25-1:100, the epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Use of acetone fixation for frozen sections is recommended. Optimal dilutions to be determined by the researcher. IHC Positive Control Tissues: Human tonsil, thymus, liver, spleen or kidney ELISA Matched Pair: Capture Antibody: Cat. #V2700-01J Detection Antibody: Cat. #215209 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice