215165-BIOTIN
TIM-1 (T-Cell Immunoglobin Mucin-1, KIM1, TIM1, TIMD1, HAVCR1) (Biotin)
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£819.00
- SKU:
- 215165-BIOTIN
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Full-length mouse Tim-1-Ig, containing both the IgV and mucin domains of Tim-1.
- Klon:
- RMT1-10
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Weitere Details:
- Tim-1 (T-cell immunoglobulin mucin 1), is a cell surface glycoprotein and member of the immunoglobulin superfamily, which was first identified as the hepatitis A virus cellular receptor (HAVCR), and is highly expressed in the liver (isoform Tim-1a) and the kidneys (isoform Tim-1b). Tim-1 is highly expressed by activated CD4+ T cells, and acts as a positive/negative co-stimulatory molecule of T cell proliferation, cytokine production and tolerance abrogation, and is the receptor for Tim-4, expressed on APCs. Tim-1 expression is greater on Th2 than Th1 cells, and studies have shown that interaction between Tim-1 on Th2 cells and Tim-4 on dendritic cells (DCs), enhances Th2 cell function, and have implicated Tim-1 as a critical player in the development of atopic disease, and in particular airway hypersensitivity. Polymorphic forms of Tim-1 are associated with increased susceptibility to asthma, eczema and Rheumatoid arthritis. TIM-1 has been shown to promote Th2 responses, and inhibit antigen-specific T cell proliferation, in contrast to the agonistic function of many Tim-1 antibodies (Xiao et al. 2007). TIM-1 has been shown to reduce the severity of EAE (experimental autoimmune encephalomyelitis) and delay disease onset, in mice studies (Xiao et al. 2007). Applications: Suitable for use in Immunofluorescence, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: Lymph node cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice