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215132-ML490

Siglec 9 (Sialic Acid-binding Ig-like Lectin 9, Siglec 9, Protein FOAP-9, UNQ668, PRO1302) (MaxLight 490)

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£837.00

SKU:
215132-ML490
Reinheit:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Lagerbedingungen:
4°C Do Not Freeze
Hersteller:
United States Biological
Host:
Mouse
Immunogen:
Recombinant protein corresponding to Siglec-9 fused to Fc region of human IgG.
Klon:
K8
Format:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Weitere Details:
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Siglec-9 (Sialic acid-binding Ig-like lectin 9), is a single pass type I membrane protein belonging to the immunoglobulin superfamily. Human Siglec-9 is broadly expressed in a range of human tissues with high expression on monocytes and low-level expression on neutrophils and subpopulations of NK, B, and T cells. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200, 10ul labels 1x10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Versandbedingungen:
Blue Ice