215041-BIOTIN
N-Cadherin (CDHN, NCAD, Neural Cadherin, CD325) (Biotin)
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£960.00
- SKU:
- 215041-BIOTIN
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.
- Klon:
- 13A9
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Weitere Details:
- Neural cadherin (N-Cadherin), otherwise known as CD325 is a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry. N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells. The clone studies have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999). The clone has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarci. The product detects a band of ~35kD-140kD in human HT-1080 and HeLa cell lysates. Applications: Suitable for use in Western Blot, Immmunohistochemistry, Immunfluorescence, Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen and Paraffin. Paraffin requires heat treatment for antigen retrieval. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice