215013-FITC
MHC Class II DR (FITC)
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£947.00
- SKU:
- 215013-FITC
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Klon:
- CC108
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
- Weitere Details:
- MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma. The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200. 10ul labels 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice