Interferon gamma, Recombinant, Human (IFN gamma)

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£754.00

SKU:: 214919
Molekulargewicht:: 16.8
Reinheit:: ≥98% (SDS-PAGE, HPLC). Endotoxin: ≤1EU/ug
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with 100ul sterile ddH2O. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Weitere Details:: IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells, and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma is species-specific and is biologically active only in human and primate cells. Recombinant human IFN-gamma is a 16.8kD protein containing 144 amino acid residues. Recombinant protein corresponding to human Interferon gamma expressed in E. coli. Swiss/UniProt Accession: P01579 Molecular Weight: ~16.7kD (143aa) Biological Activity: The ED50 determined by a cytotoxicity assay using HT-29 cells is ≤0.05ng/ml, corresponding to a specific activity of ≥2x10e7 units/mg. Amino Acid Sequence: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice