214885-ML650
HIF1 Alpha (Hypoxia-Inducible Factor 1 alpha) (MaxLight 650)
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£927.00
- SKU:
- 214885-ML650
- Reinheit:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human HIF-1 Alpha.
- Klon:
- Halpha111a
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Hypoxia-inducible factor 1-alpha otherwise known as HIF1 alpha (HIF1A) or ARNT interacting protein, a widely expressed bHLH-PAS transcription factor, which acts as a critical regulatory protein in the host response to hypoxia (Kubis et al. 2005). HIF1A and HIF1B form the highly-conserved heterodimeric HIF-1 transcriptional complex (Carmeliet et al. 1998). During hypoxic conditions, HIF1A accumulates in the nucleus and activates the transcription of many genes encoding proteins involved in the production of oxygen delivery and metabolic adaptation, such as vascular endothelial growth factor and glucose transporters. Under normal oxygen conditions, HIF1A is targeted by HIF prolyl-hydroxylases, followed by rapid protease degradation, which is inhibited during hypoxia (Hirsilä et al. 2003). Overexpression of HIF1A occurs in many common human cancers, including pancreatic, bladder and renal carcinomas (Shibaji et al. 2003). Mouse anti Human HIF1alpna, has been successfully used for the identifcation of HIF1alpha in human samples by both immunohistochemistry and Western blotting. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry(Paraffin): Requires antigen retrieval using heat treatment using Tris/EDTA buffer pH 9.0 prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice