214615-FITC
CD25 (IL-2R Alpha Chain, Interleukin-2 Receptor Alpha Chain) (FITC)
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£819.00
- SKU:
- 214615-FITC
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Cell membrane preparation of HTLV-1 transformed rabbit T cell line.
- Klon:
- KEI-alpha1
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
- Weitere Details:
- CD5, a 55kD cell surface antigen, otherwise known as the interleukin-2 (IL-2) receptor alpha chain. In rabbits, CD25 is expressed on activated T lymphocytes. Mouse anti Rabbit CD25 antibody, is reported to inhibit the binding of IL-2 to its receptor (Kotani et al. 1993). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: PAI myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice