209158
Protein G, Recombinant
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£767.00
- SKU:
- 209158
- Molekulargewicht:
- 2332
- Reinheit:
- Highly Purified (~95%)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a lyophilized powder in salt free form. Reconstitute with sterile ddH2O or PBS.
- Weitere Details:
- Protein G is a bacterial cell wall protein isolated from group G Streptococci. Native Protein G contains two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from purified Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also good at pH 7.0-7.2. Protein G has greater affinity than Protein A for most mammalian IgGs, it may be used for the purification of mammalian IgGs that do not bind well to Protein A. Protein G does not bind to human IgM, IgD and IgA. Native protein is ~65kD. It contains two albumin binding domains at the N-terminus and 3 IgG binding domains (~55aa each) at the C-terminus. Recombinant protein G does not contain albumin binding domains. Differences in binding characteristics between Protein A and Protein G may be explained by the differing compositions in the IgG-binding sites of each protein, and sequence differences between Protein A and Protein G. Variations in isolation and purifications methods for Protein G may affect IgG binding, partially because there are differing numbers of IgG-binding sites on various sources of Protein G. Source: Recombinant protein corresponding to Protein G from Staphylococcus Group G, expressed in E. coli. Molecular Weight: ~23kD, migrates to ~32kD protein Binding Capacity: 1mg binds 10-15mg of human IgG. Binding of other species may be different. Endotoxin: <0.5EU/mg protein Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice