208919
Glutamic Acid Decarboxylase 67, Recombinant, Mouse, His-Tag, Positive Control for Western Blot (GAD67, GAD1)
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£576.00
- SKU:
- 208919
- Anwendung:
- WB
- Molekulargewicht:
- 67
- Reinheit:
- Highly Purified (~95%)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied in SDS-PAGE sample buffer (reduced). Add 5ul of fresh 2x sample buffer/10ul of the solution prior to heating and loading on gels.
- Weitere Details:
- Gamma Aminobutyric acid (GABA) is the major known inhibitory neurotransmitter. The rate-limiting step in the synthesis of GABA is the decarboxylation of glutamate by glutamate decarboxylase (GAD; L-glutamate 1-carboxy-lyase, EC 4.1.115). In the CNS GAB is entirely restricted to GABAergic neurons. GAD is also present in the b-cells of the pancreas and autoantibodies to various GAD polypeptides are detected in insulin-dependent diabetes mellitus. Cloning of GAD genes have identified two subtypes: GAD65 (65kD; human 585aa chromosome 10) and GAD67 (67kD; human 594aa, chromosome 2) share approx. 65% amino acid homology. The N-terminus is the most divergent while the C-terminus is highly conserved. Although both GAD isoforms catalyzes the conversion of GABA but interact differently with the co-factor pyridoxal 5'- phosphate suggesting their activities are differentially regulated. GAD67 is cytosolic, while GAD65 is membrane associated. GAD65 is a major autoantigen in diabetes mellitus and stiff-man syndrome, a rare disease of the brain. Source: Recombinant protein corresponding to full-length mouse GAD67, fused to His-tag, expressed in E. coli. Molecular Weight: ~67kD Applications: Suitable for use as Positive Control for Western Blot. It is not suitable for ELISA or other applications where native protein is required. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice