208716

Acrp30, Recombinant, Mouse, Trimeric Form (Adiponectin, Adipocyte Complement-related Protein of 30kD, AdipoQ, APM1, Adiponectin, Gelatin binding protein 28kD, GBP28, Adipocyte Most Abundant Gene Transcript)

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£832.00

SKU:: 208716
Reinheit:: ≥95%
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a lyophilized powder in PBS, pH 7.4. No preservative added. Reconstitute with sterile PBS, 0.1% protease-free BSA.
Weitere Details:: Acrp30 (Adipocyte complement-related protein of 30kD), also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28kD/GBP28 or adipocyte most abundant gene transcript) was identified as a novel adipocyte-specific synthesized. It is a secreted protein with structural resemblance to complement factor C1q. Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans. Low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased Acrp30 levels. Acrp30 (mouse 247aa, rat and human 244aa; chromosome 3q27) consists of a predicted NT-signal sequence 91-14aa); followed by a 27aa unique region; followed by 22 perfect Gly-X-Pro or Gly-X-X collagen like repeats; and a globular segment at the C-terminus. Structurally, at the sequence level. Acrp30 resembles other collagen-like and globular domain proteins (lung surfactant protein and hepatocytes mannan-binding proteins). Acrp30 is proteolytically cleaved at 104aa to generate the globular Acrp30 (gAcrp30). Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss. A marked reduction in plasma triglycerides, glucose and free fatty acids was attributed due in part to increased fatty acid oxidation by muscle. Full length Acrp30 was less potent than gAcrp30. Therefore, gAcrp30 may open new avenues to control obesity. Recombinant protein corresponding to the Trimeric form of mouse Adiponectin, expressed in HEK293 cells, stably transfected with pchAd-Fvector encoding FLAG epitope-tagged proteins. Biological Activity: ED50=3-8.5g/ml, as determined by its ability to inhibit proliferation of HASMCs induced by HB EGF. Applications: Suitable for use in In vitro, Ex vivo, In vivo Activity Analysis, Binding assay and ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% protease-free BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice