208705
Transforming Growth Factor beta1, Recombinant, Human, Animal Free (TGF-beta1, Differentiation Inhibiting Factor, Cartilage-Inducing Factor)
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£351.00
- SKU:
- 208705
- Reinheit:
- ≥98% (SDS-PAGE gel, HPLC). Endotoxin: ≤0.1ng/ug (<1EU/ug)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a lyophilized powder. No additives. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml.
- Weitere Details:
- The three mammalian isoforms of TGF-b, TGF-b1, b2, b3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-b isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-b1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Recombinant human TGF-b1 is a 25kD protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond, expressed in CHO cells. Biological Activity: Determined by TGF-b1’s ability to inhibit the mouse IL-4- dependent proliferation of mouse HT-2 cells. The expected ED50 is ≤0.05ng/ml, corresponding to a specific activity of >2x10e7units/mg. Amino Acid Sequence: ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice