207576
DUX4 (DUX10, Double Homeobox Protein 4, Double Homeobox Protein 10) (ATTO 488)
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£846.00
- SKU:
- 207576
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant GST fusion protein corresponding to a 76aa fragment of human DUX4 at C-terminus.
- Klon:
- P2B1
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol. Labeled with ATTO 488.
- Weitere Details:
- This gene is located within a D4Z4 repeat array in the subtelomeric region of chromosome 4q. The D4Z4 repeat is polymorphic in length; a similar D4Z4 repeat array has been identified on chromosome 10. Each D4Z4 repeat unit has an open reading frame (named DUX4) that contains two homeoboxes; the repeat-array and ORF is conserved in other mammals. There was no evidence for transcription from standard cDNA libraries however RTPCR and in-vitro expression experiments indicate that the ORF is transcribed and the encoded protein has been reported to function as a transcriptional activator of paired-like homeodomain transcription factor 1 (PITX1; GeneID 5307). Contraction of the microsatellite repeat causes autosomal dominant facioscapulohumeral muscular dystrophy (FSHD). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot (transfected lysate): 1:1000 Optimal dilutions to be determined by the researcher. Extinction Coefficient: 90,000 Excitation (Max): 501nm Emission (Max): 523nm Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. ATTO 488 conjugates are sensitive to light.
- Versandbedingungen:
- Blue Ice