207414
TDO, Recombinant, Anopheles gambiae, His-Tag (TPH2, TRPO, Tryptophan 2,3-dioxygenase, Tryptophan Oxygenase, Tryptophan Pyrrolase, Tryptophanase)
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£619.00
- SKU:
- 207414
- Molekulargewicht:
- 47.9
- Reinheit:
- ≥98% (SDS-PAGE)
- Lagerbedingungen:
- -70°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 8.0, 0.15M sodium chloride, 0.2M imidazole, 10% glycerol.
- Weitere Details:
- Tryptophan 2,3-dioxygenase (TDO) is a heme-containing dioxygenase catalyzing the addition of molecular oxygen across the 2,3-double bond of the indole ring of tryptophan to form N-formylkynurenine (NFK). In Anopheles gambiae, TDO is the only enzyme able to catalyze the first and rate-limiting step in L-Trp catabolism through the kynurenine pathway. Tryptophan is an essential amino acid for protein synthesis and also the precursor for production of a number of neurotransmitters, such as serotonin and melatonin; in mosquitoes, the kynurenine pathway is essential for eye pigmentation. Conceivably, the tryptophan-using pathways should be regulated in a coordinated manner in mosquitoes as well as in other species and TDO activation/ inactivation processes could play an essential role in these phenomena. Recombinant protein corresponding to full-length Anopheles gambiae TDO, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~47.9kD Applications: Can be used to test potential kynurenine pathway inhibitors in the mosquito, by continuously monitoring the rate of NFK formation at 321nm (E=3152/Mcm) in in vitro spectrophotometric assays. Biological Activity: Specific activity towards L-Trp: 87umol/hr/mg. TDO catalyzes the oxidative opening of the L-tryptophan indole moiety to yield N-formylkynurenine (NFK). Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Dry Ice