207305

NMNAT3, Recombinant, Human, aa1-252, His-Tag, Highly Active (Nicotinamide Mononucleotide Adenylyltransferase 3, NMN Adenylyltransferase 3, Nicotinate-nucleotide Adenylyltransferase 3, NaMN Adenylyltransferase 3, Pyridine Nucleotide Adenylyltransferase 3, PNAT-3, FKSG76)

Größe

£369.00

SKU:: 207305
Molekulargewicht:: 27
Reinheit:: ~95% (SDS-PAGE)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 50mM TRIS-HCl, pH 8.0, 300mM sodium chloride, 1mM DTT, 10% glycerol.
Weitere Details:: Mitochondrial NMNAT isoform. Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Can use tiazofurin monophosphate as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, mitochondrial NMNAT isoform can use NAD(+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. Protects against axonal degeneration following injury. Recombinant protein corresponding to full-length human NMNAT3, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~27kD (monomer) Applications: Well suited for the synthesis of NAD analogs due to lower substrate selectivity compared to NMNAT1. For NAD synthesis use NMNAT1. Biological Activity: ≥2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1umol of NAD+ per minute. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice