172112-AF
IgM (AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region, Immunoglobulin Mu Heavy Chain) (BSA & Azide Free)
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£646.00
- SKU:
- 172112-AF
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant heavy chain of human IgM. Cellular Localization: Cytoplasm, Cell Surface and Secreted
- Klon:
- SPM557
- Format:
- Supplied as a liquid in PBS. Also available with BSA and azide, see 172112.
- Weitere Details:
- Recognizes a protein of 75kD, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml Immunohistochemistry (Formalin fixed and paraffin embedded tissues): 0.5-1ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice