172102-AF
gp100 (95kD melanocyte-specific Secreted GlycoProtein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte Protein Pmel 17, Melanoma Associated ME20 antigen, Melanosomal matrix Protein17, p100, p26, PMEL17, Premelanosome Protein, Secreted melanoma-Associated ME20 antigen, SILV, Silver Homolog, Melanosome) (BSA & Azide Free)
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£646.00
- SKU:
- 172102-AF
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Membranes from a human melanoma metastasis. Cellular Localization: Cytoplasmic
- Klon:
- SPM286
- Format:
- Supplied as a liquid in PBS. Also available with BSA and azide, see 172102.
- Weitere Details:
- By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Positive Control: SK-MEL-28 cells or Melanoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice