172080-AF-PE

Cytokeratin 8 (CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8) (PE)

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£876.00

SKU:: 172080-AF-PE
Reinheit:: Purified by Protein A/G affinity chromatography.
Lagerbedingungen:: 4°C Do not freeze
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Cytoskeleton preparation containing cytokeratin 8. Cellular Localization: Cytoplasmic
Klon:: SPM538
Format:: Supplied as a liquid in PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).
Weitere Details:: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (“ring-like, perinuclear”) from ductal (“peripheral-predominant”) carcinoma of the breast. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Versandbedingungen:: Blue Ice