172035-AF-BIOTIN
Biotin (Vitamin B7, Vitamin H) (Biotin)
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£876.00
- SKU:
- 172035-AF-BIOTIN
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Biotinylated sheep immunoglobulin. Cellular Localization: Depends on the target
- Klon:
- SPM375
- Format:
- Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
- Weitere Details:
- It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml In situ Nucleic Acid Hybridization; Western Blotting; Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.) Optimal dilution for a specific application should be determined. Positive Control: Biotinylated proteins in solution or on tissues Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice