171920

MEKK2, Active, Recombinant, Human

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£918.00

SKU:: 171920
Molekulargewicht:: 96.5
Reinheit:: Purified (~87%)
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 3mM DTT, 20% glycerol.
Weitere Details:: Recombinant human MEKK2, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system. Source: Full length recombinant protein corresponding to human MEKK2 at N-terminal, fused to GST-tag expressed in Sf9 insect cells. Molecular Weight: ~96.5kD Specific Activity: 18pmol/min/ug Assay Conditions: Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 uM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 minutes at 30°C. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.