NEK9 (Kinase Domain) (KIAA1995, NERCC, NERCC1, Nercc1 Kinase, NIMA-related Kinase 8, NIMA-related Kinase Nek8, NIMA-related Kinase 9, NimA-related Protein Kinase 9)

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£735.00

SKU:: 170997
Anwendung:: IHC-Fr
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 20aa from human NEK9. Species sequence homology: canine, xenopus and rat.
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Weitere Details:: NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis. The protein has a N-terminal NIMA-like catalytic domain, a central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1), and a C-terminal coiled-coil domain. It is phosphorylated by active p34(Cdc2) and is capable of autophosphorylation and oligomerization. The activated protein binds Nek6 and RCC1, and has been shown to associate with the human homolog of the Drosophila protein Bicaudal D (Bicd2) in vivo and to phosphorylate this coiled-coil protein in vitro. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.