168861-AF

SUMO-1 (GAP-modifying protein 1, GMP1, OFC10, PIC1, SENP2, Sentrin 1, Small ubiquitin-related modifier 1, SMT3, SMT3 suppressor of mif two 3 homolog 1, SMT3C, SMT3H3, Ubiquitin homology domain protein PIC1, Ubiquitin Like 1, Ubiquitin like protein UBL1) (BSA & Azide Free)

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£646.00

SKU:: 168861-AF
Reinheit:: Purified by Protein A/G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human SUMO-1 protein. Cellular Localization: Predominantly nuclear with some cytoplasmic
Klon:: SM1/495
Format:: Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168861.
Weitere Details:: The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1-2ug/million cells Immunofluorescence: 1-2ug/ml Western Blot: 1-2ug/ml for 2 hours at RT Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: Breast carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice