168855-AF-BIOTIN
Prolactin Receptor (CPRLP, hPRL receptor, hPRLrI, Lactogen receptor, OPR, Pr-1, Pr-3, PRL-R, Prolactin receptor delta 7/11, RATPRLR, Secreted prolactin binding protein, Truncated testis-specific box 1-C prolactin receptor) (Biotin)
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£876.00
- SKU:
- 168855-AF-BIOTIN
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Semi-purified human prolactin receptor. Cellular Localization: Cell surface and cytoplasmic
- Klon:
- B6.2
- Format:
- Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
- Weitere Details:
- It recognizes a protein of 70kDa, identified as prolactin receptor. Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor), which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription. Applications: Suitable for use in Immunofluorescence, ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml Western Blot: 0.25-0.5ug/ml Immunoprecipitation: 0.5-1ug/500ug protein lysate Immunohistochemistry: Frozen & Formalin-fixed: 0.5-1ug/ml for 30 minutes at RT (Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C. Optimal dilutions to be determined by the researcher. Positive Control: Breast, placenta, liver or pancreas Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice