168841-AF
MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (BSA & Azide Free)
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£646.00
- SKU:
- 168841-AF
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant mouse MyoD1 protein (5.8A) and Recombinant human MyoD1 protein (MYD712). Cellular Localization: Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentatiation.
- Klon:
- 5.8A, MYD712
- Format:
- Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168841.
- Weitere Details:
- MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm’s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing’s sarcomas and alveolar soft part sarcomas. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/million cells Immunohistochemistry (formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Requires boiling tissue sections in 10mM Tris, 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Rhabdomyosarcoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice