Ku (p70/p80) (Ku (p70): 70kD subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70kD subunit, CTC box-binding factor 75kD subunit, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, Ku autoantigen, 70kD, Ku70, Kup70, Lupus Ku autoantigen protein p70, ML8, Thyroid autoantigen 70kD (Ku antigen), Thyroid-lupus autoantigen (TLAA), X-ray repair cross-complementing protein 6 (XRCC6) Ku (p80): 86kD subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 2, ATP dependent DNA helicase II 86kD subunit, ATP-dependent DNA helicase II 80kD subunit, CTC box-binding factor 85kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, KARP1, Ku autoantigen 80kD, Ku80, Ku86 autoantigen related protein 1, KUB2, Lupus Ku autoantigen protein p86, Nuclear factor IV (NFIV), Thyroid-lupus autoantigen (TLAA), X-ray repair cross-complementing protein 5 (XRCC5))

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£422.00

SKU:: 168818
Reinheit:: Purified by Protein A/G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Nuclear extract of human HL-60 cells. Cellular Localization: Nuclear
Klon:: KU729
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 168818-AF.
Weitere Details:: Recognizes a dimer of two proteins of 70kD and ~80kD, identified as two subunits of Ku. 168818 recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western Blot. The p70/p80 dimer is important for function of a 460kD DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation and apoptosis. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1-2ug/ml Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT Flow Cytometry: 1-2ug/million cells Optimal dilutions to be determined by the researcher. Positive Control: Human cancer K562, HL-60, HEp-2 or HeLa cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice