168782-AF
gp100 (Melanosome, 95kD melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog) (BSA & Azide Free)
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£646.00
- SKU:
- 168782-AF
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Extract of pigmented melanoma metastases from lymph nodes. Cellular Localization: Cytoplasmic
- Klon:
- HMB45
- Format:
- Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168782.
- Weitere Details:
- Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. Applications: Suitable for use in ELISA, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 Immunofluorescence: 0.5-1ug/ml Immunohistochemistry (Formalin-fixed): 0.5-1.0ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: SK-MEL-28 cells or Melanoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice