168769-AF-FITC

Ep-CAM (CD326, Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TACSTD1, TROP1, Tumor-associated Calcium Signal Transducer 1, ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, M17S1 (Membrane Component, Chromosome 17, Surface Marker-1), REG-1, Reggie-1, Reggie-2) (FITC)

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£876.00

SKU:: 168769-AF-FITC
Reinheit:: Purified by Protein A/G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: MCF-7 human breast cancer cells. Cellular Localization: Cell surface
Klon:: 323/A3
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).
Weitere Details:: EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: non-reducing conditions only Immunohistochemistry (FFPE): Staining of formalin/paraffin tissues requires boiling of tissue sections with 10mM citrate buffer, pH 6, for 10-20 minutes followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: HT29 cells or breast tumor Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Versandbedingungen:: Blue Ice