Cytokeratin 19 (k19, k1cs, Keratin 19 Keratin Type i 40kD, krt19)

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£422.00

SKU:: 168749
Reinheit:: Purified by Protein A/G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Human breast cancer MCF-7 cells (A53-B/A2); Human mammary epithelial organoids (BA17). Cellular Localization: Cytoplasmic
Klon:: A53-B/A2.26, BA17
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide.
Weitere Details:: This Ab reacts with the rod domain of human cytokeratin 19 (CK19), a polypeptide of 40kD. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (formalin-fixed): 0.5-1ug/ml for 30 min at RT Requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: MCF-7, HeLa cells, Hep-G2 cells, Breast Cancer Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice