168666-AF-PE
CD147 (EMMPRIN, Neurothelin, 5A11 Antigen, 5F7, Basigin, Blood brain barrier HT7 antigen BSG, Extracellular Matrix Metalloproteinase Inducer (EMMPRIN), Leukocyte Activation Antigen M6, Neurothelin, OK Blood Group Antigen, TCSF, Tumor Cell-derived Collagenase Stimulatory Factor) (PE)
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£876.00
- SKU:
- 168666-AF-PE
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human T cell line Molt 13. Cellular Localization: Cell surface
- Klon:
- 8D6
- Format:
- Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).
- Weitere Details:
- This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1:100-1:200 Immunohistochemistry: Frozen & Formalin-fixed: 1-2ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: HSB2 cells. Colon or breast carcinoma Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice