168644
AMACR (Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM, p504S)
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£448.00
- SKU:
- 168644
- Reinheit:
- Culture Supernatant
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Full length human recombinant AMACR protein. Cellular Localization: Cytoplasmic
- Klon:
- 13H4
- Format:
- Supplied as liquid, 0.05% sodium azide.
- Weitere Details:
- This antibody recognizes a protein of 54kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and b-oxidation of branched-chain fatty acids. AMACR is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:200 for 2 hours at RT Immunohistochemistry: formalin-fixed: 1:50-1:100 for 30-60 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: HEK cells or Prostate Adenocarcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice