SGK (SGK1, Serine/Threonine-protein Kinase SGK1, Serum/Glucocorticoid-regulated Kinase 1)

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£868.00

SKU:: 168505
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the C-terminal region near aa400-431 from human SGK-1. Species Sequence Homology: mouse, rat, canine, chicken, bovine, rabbit and spiny dogfish.
Format:: Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Weitere Details:: SGK-1 (also called Serine/threonine-protein kinase Sgk1 and Serum/glucocorticoid-regulated kinase 1) is a protein kinase that plays an important role in cellular stress response. SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. This kinase mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A and phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, Kv1.3, or EAAT1. SGK1 is localized to the cytoplasm and upon phosphorylation is translocated to the nucleus. The kinase is expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Induction occurs upon exposure to glucocorticoids and by excessive extracellular glucose or TGF-b, in cultured cells. SGK-1 is regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at 422aa which in turn increases the phosphorylation of 256aa by PDPK1. The kinase is ubiquitinated by NEDD4L; which promotes proteasomal degradation. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:4000-1:16,000 Western Blot: 1:500-1:3000 Immunohistochemistry (paraffin): 10-20ug/ml using heat induced antigen retrieval in pH 6.0 citrate buffer. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice