168213
Gli-3 (Gli-3, Zinc Finger Protein Gli-3, GLI-Kruppel Family Member GLI-3)
Größe
£914.00
- SKU:
- 168213
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to an internal region near aa30-60 of human Gli-3 Species Sequence Homology: chimpanzee, squirrel monkey, xenopus laevis, chicken, canine, mouse, rat and quail.
- Format:
- Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
- Weitere Details:
- Gli-3 (also known as zinc finger protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and cranio-facial development. Two isoforms of human Gli-3 have been reported. One is the full-length protein at~170-190kD and the other is a truncated isoform at~80kD. Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates. Applications: Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): 0.5-5ug/ml, Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. ELISA: 1:6000-1:30,000 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice