167885
IL-1 beta, Recombinant, Human
Größe
£524.00
- SKU:
- 167885
- Molekulargewicht:
- 17.3
- Reinheit:
- ≥98% (HPLC, SDS-PAGE). Endotoxin: ≤0.1ng/ug
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a lyophilized powder. Reconstitute with dH2O to 0.1-1mg/ml following centrifugation.
- Weitere Details:
- IL-1beta is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant human IL-1b is a 17.3kD protein containing 153aa residues. Recombinant protein corresponding to human IL-1b expressed in E.coli. Biological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells is ≤1pg/ml, corresponding to a specific activity of ≥1x10e9 units/mg. Amino Acid Sequence: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice